MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



mCKSON'S PURPLE 

 A general stain suitable for class work 



Solution required: 

 Hickson purple, saturated aqueous. 



Technique: 



1 . Bring sections down to water as usual. 



2. Stain in Hickson's purple for ten to twenty minutes. 



3. Dehydrate, clear and mount. 



Results: 



Leucocytes, purple ; erythrocytes, distinct red. The rest of the 

 tissues purple. 



Reference: Cannon, H. G. (1941),^- Roy. Micr. Soc, series III, 61, parts 3 

 and 4. 



HICKSON'S PURPLE - VICTORIA GREEN 

 A general stain, particularly suitable for class work 



Solutions required: 



A. Hickson's Purple saturated aqueous. 



B. Victoria green, G. saturated in 70% alcohol. 



Technique: 



1 . Fix sections to slides ; dewax and take through the alcohols 

 down to distilled water as usual. 



2. Stain for ten minutes in the Hickson's purple. 



3. Rinse in distilled water. 



4. Stain in the victoria green for half to one hour. 



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