SECTION TWO 



5. Rinse in 70%, followed by 90% alcohol. 



6. Clear in xylol and mount. 



Results: 



Nuclear are sharply defined, purple. Erythrocytes sharply 

 defined, stained vivid green, against a general blue-purple back- 

 ground. 



Note: This method is an improvement of Hickson purple used 

 alone. 



Reference: Cannon, H. G. (1941), J. Roy. Micr. Soc, series III, 61, parts 3 

 and 4. 



fflTCHCOCK AND EHRLICH'S MIXTURE 



For lymphatic, ganglion, plasma and basophilic cells; im- 

 mature cells of bone marrow, striated muscle, and fibrin. 



Technique: 



1. Fix in Zenker-acetic acid, corrosive sublimate, but not in 

 Miiller or formalin. 



2. Paraffin sections are brought down to 90% alcohol; then 

 passed through a solution of iodine in 90% alcohol. 



3. The iodine is removed by passing through the graded 

 alcohols to water and finally washing for fifteen minutes in running 

 water. 



4. Flood with the stain and allow it to act for fifteen to thirty 

 seconds ; then pour oif the stain and wash rapidly in water. 



5. The preparation is then passed directly into absolute alcohol, 

 where it is allowed to remain only as long as the stain continues to 

 be washed out in clouds. 



6. Clear in xylol and mount. 



Note: Sometimes the brilliancy of the stain is enhanced by 

 re-staining. 



Results: 



Plasma cells: cytoplasm, brilliant crimson: nuclei, bluish- 

 green. Other cells appear in lighter shades of green and 

 crimson. 



131 



