MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



JENNER STAIN 

 For blood-forming organs 



B. Jenner stain. 



Technique: 



1 . Fix pieces of tissue for two or three days in Solution A. 



2. Dehydrate in ascending grades of alcohol as usual; clear; 

 embed in paraffin wax. 



3. Fix sections, not exceeding 5/z in thickness, to slides ; de-wax ; 

 pass through the usual descending grades of alcohol down to dis- 

 tilled water which has been buffered to pH 7-0. 



4. Stain for forty-five minutes in a grooved, stoppered staining 

 jar, with a mixture consisting of equal volumes of Jenner stain 

 and distilled water, buffered to pH 7.0. 



5. Differentiate and dehydrate with absolute alcohol. 



6. Clear in xylol and mount in Cristalite. 



Results: 



Neutrophile granules are stained pink. Oxyphile granules, 

 brownish red. Basophile granules, purple. Nucleoli (plasmosomes), 

 pink. The cytoplasm of partially haemoglobinated precursors of 

 erythrocytes are stained in varying shades of reddish violet, while 

 mature erythrocytes are deep pinkish orange. 



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