MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



13. Clear in xylol and mount in Cristalite. 



Results: 



Erythroc3rtes are stained orange. Cytoplasm of lymphocytes 

 and blastocytes are blue. Nuclei, deep blue to violet. Mast cell 

 granules, violet to violet-red. 



LEAD HAEMATOXYLIN - ACID FUCHSIN (MacConaill) 



A " definitive '* polychrome stain for the central nervous 

 system and the trunks outside it. 



The essence of this technique, which is due to Professor M. A. 

 MacConaill, of the Department of Anatomy, University College, 

 Cork, Ireland, is that the lead haematoxylin reduces the ammon- 

 ium molybdate to form a blue lake, which makes it possible to 

 employ only the minimum exposure to haematoxylin, thereby 

 leaving the erythrophile (*' Fuchsinophile ") parts of the neurone 

 red. 



Solutions required: 



A. Lead nitrate 

 Glacial acetic acid 

 Acid fuchsin 

 Water 



B. Haematoxylin 

 Acetic acid 4% aqueous 



C. Solution A 

 Solution B 



2gm. 

 8 ml. 

 0-5 gm. 

 92 ml. 



I gm. 

 100 ml. 



I volume 

 I volume 



Note: This solution should be prepared as and 

 when required : it deteriorates after one day. 



D. Liquor ammon. acetat. B.P. . . 10 ml. 

 Ammonium molybdate, saturated, 



aqueous.. .. .. .. 70 ml. 



Water . . . . . . . . 80 ml. 



Note: All the above solutions must be made with- 

 out the application of heat. Tap water may be used : 

 the solutions must be filtered. 



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