SECTION TWO 



Results: 



Collagen is stained a reddish purple, while reticulum appears as 

 black threads. 



LORRAIN - SMITH - DIETRICH STAIN 



For lipoids 



Solutions required: 



A. Potass, dichromate 5% aqueous. 



Haematoxylin (Kultschitzky) 



B. Haematoxylin 10% in absolute 



alcohol (ripened three months 



or longer) . . . . . . 10 ml. 



Acetic acid 2% aqueous . . 90 ml. 



C. Potass, ferricyanide .. .. 2-5 gm. 

 Borax 2% aqueous . . . . 100 ml. 



Technique: 



Material is fixed in 10% formalin and frozen sections are em- 

 ployed. 



1. Mordant sections twenty-four to forty-eight hours in Solu- 

 tion A at 37° C. ; then wash thoroughly in distilled water. 



2. Immerse in Solution B at 37° C. for four to five hours; then 

 wash in distilled water. 



3. Differentiate overnight in Solution C. 



4. Wash thoroughly in distilled water; drain and mount in 

 Aquamount or in Farrant. 



Results: 



Lipoid substances, blue-black. 



LUGOL'S IODINE 

 For the identification of glycogen in tissues 



Solution required: 

 Lugol's iodine. 



143 



