MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



(b) Paraffin sections 



1. Cut sections 15 to 20jLt in thickness and fix to slides. 



2. Remove paraffin wax with xylol and pass through absolute 

 alcohol. 



3. Rinse with several changes of 95% alcohol. 



4. Stain with the Luxol fast blue solution for five to twenty-four 

 hours, but preferably not less than sixteen hours, at 57° C. in an 

 oven, taking precautions to prevent the loss of alcohol through 

 evaporation from the staining solution. 



5. Proceed exactly as at Stage 4 in the technique given above 

 for frozen sections, except at Stage 1 1 the cresyl violet should be 

 allowed to act for six minutes. 



(c) Celloidin sections (loose) 



1. Cut sections 15 to 30/z in thickness and place them into 75% 

 alcohol. 



2. Stain from five to twenty-four hours, but preferably not less 

 than sixteen, in the Luxol fast blue solution at 57° C. in an oven, 

 taking precautions to prevent the loss of alcohol by evaporation 

 from the staining solution. 



2. Proceed exactly as at Stage 4 in the technique given for 

 frozen sections, except at Stage 1 1 the staining time for the cresyl 

 fast violet should be increased to three minutes. 



(d) Celloidin sections (affixed) 



1. Cut sections 15 to 30jLt in thickness, keeping the microtome 

 knife and tissue continually flooded with 75% alcohol. 



2. Place sections on slides, which have previously been smeared 

 with glycerine albumen. 



3. If necessary flatten out the sections en the slides by rolling 

 with a piece of glass tubing half an inch in diameter and about two 

 inches in length, or a small glass phial will serve the purpose. 



4. Drop on sufficient clove oil to cover the sections and leave 

 the oil to act for five minutes. 



5. Remove the clove oil with 95% alcohol. 



6. Remove the Celloidin with absolute alcohol. 



7. Wash with 95% alcohol. 



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