SECTION TWO 



8. Proceed exactly as at Stage 2 in the technique given for 

 frozen sections, except at Stage 2 the staining time for cresyl fast 

 violet should be increased to six minutes. 



Results: 



Myelinated fibres are sharply contrasted greenish-blue against 

 the reddish-coloured Nissl cells. The technique shows the Nissl 

 picture and differentiates between the three types of glia cells: 

 Myelin sheaths, neurons and glia nuclei are well demonstrated. 

 Differentiation is also obtained between the three layers of 

 medium-sized and larger blood cells, and capillary endothelium as 

 well as mesothelial lining of Arachnoid membrane are sharply 

 outlined. The finer fibres of the molecular layer of the cerebral 

 cortex can be most effectively demonstrated in paraffin sections. 

 Bacteria and pigments in nerve cells are more clearly demonstrated 

 with this technique than with the usual Nissl stains. 



References : 

 Kliiver, Heinrich and Barrera, Elizabeth (1953), J. of Neuropath and Exp. 



Neurology, 12, no. 4, 400-3, " A Method for the combined staining of cells 



and fibres in the nervous system ". 

 Kliiver, Heinrich and Barrera, Elizabeth (1954), X of Psychology, yj, 199-223, 



" On the use of Azoporphin derivatives (Phthalocyanines) in the staining of 



nervous tissue ". 



Note: In the original paper it is stated that this cell-fibre stain 

 has been employed for peripheral nerves as well as structures of the 

 central nervous system in amphibians, birds and mammals (rat, 

 guinea-pig, rabbit, cat, monkey, chimpanzee, gorilla and man), 

 and that with suitable counterstains Luxol fast blue will give ex- 

 cellent preparations of cochlea, adrenals and numerous extraneural 

 tissues. 



MacCALLUM'S STAIN 

 For influenza bacilli and Gram-positive organisms in tissues 



H7 



