SECTION TWO 



B. Harris or Ehrlich haematoxylin. 



C. Lithium carbonate, saturated aqueous. 



D. Acid Fuchsin (Mallory) o*5% aqueous. 



E. Phosphomolybdic Aniline Blue - Orange G 

 (Mallory). 



Technique: 



1. Mammalian pancreases are fixed in Bouin and afterwards 

 washed in 80% alcohol. 



2. Dehydrate, clear, and embed in paraffin wax. 



3. Cut sections 4 to 5 jit in thickness. 



4. Fix sections to slides: remove wax with xylol and pass 

 through the usual grades of alcohol down to distilled water. 



5. Immerse in solution A for about one half to one minute, until 

 the sections appear uniform reddish brown in colour. 



6. Rinse in distilled water. 



7. Stain with Ehrlich or Harris Haematoxylin for five to ten 



minutes. 



8. Blue in lithium carbonate solution. 



9. Wash well in tap water. 



10. Wash with distilled water. 



11. Stain in Mallory's Acid Fuchsin, controlling under micro- 

 scope until the A cells are red, and the B cells are pink. 



If overstained, wash out with distilled Vv^ater until the above 

 effects are obtained. 



12. Stain in solution E for twenty minutes to twelve hours 

 according to the condition of the pancreas and degree of dif- 

 ferentiation in the first stain. 



Results: 



Nuclei are stained dark violet; nucleoli, red. Cytoplasm in A 

 cells contains red granules. Cytoplasm of B cells contains blue 

 granules. Cytoplasm in acinar cells varies from red to pale violet 

 with deep violet ergastoplasm. Canahcular cells, blue-grey. 

 Connective tissue blue. Erythrocytes red. Mucus, azure blue. 



Reference: Isaac, J. P. and Aron, C. (1952), Bull. Mies. Appl. ser. 2, 2, 

 99-102. 



149 



