MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



MALLORY STAIN - HAEMATOXYLIN 



For differential staining of acidophils, basophils and 

 chromophobes in mouse pituitary 



Solutiotis required: 



A. Zenker - Formal : 



Zenker's Fluid . . . . . . 95 ml. 



Formaldehyde . . . . . . 5 ml. 



B. Formic acid 10% aqueous. 



C. Iodine 0-5% in 70% alcohol. 



D. Sodium thiosulphate 075% in 10% alcohol. 



E. Harris haematoxylin. 



F. Lithium carbonate satd. aqueous. 



G. Acid fuchsin 0-5% aqueous. 



H. Phosphomolybdic acid 1% aqueous. 

 I. Mallory's Aniline blue-orange G. 

 J. Carbol xylol. 



Technique: 



1. Pituitary gland together with bone to which it is attached is 

 fixed in solution A for 4-8 hours, or overnight in a refrigerator. 



2. Wash in running tap water for 8 to 10 hours. 



3. Decalcify by immersing in 10% formic acid for 24 hours. 



4. Wash in running tap water for 2 to 4 hours. 



5. Dehydrate, clear and embed in paraffin wax 56 to 58° C. in 

 the usual way. 



6. Cut sections about 4)Lt in thickness and mount on slides with 

 glycerine albumen. 



7. Dry the slides thoroughly in an oven at 56° C. for one to two 

 hours, or overnight at room temperature. 



8. Remove wax with xylol; then wash with two changes of 

 absolute alcohol. 



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