SECTION TWO 



9. Wash with 90% followed by 70% alcohol. 



10. Immerse for half to two minutes in solution C (Iodine). 



1 1 . Wash well with water. 



12. Immerse in solution D (thiosulphate) for half to two minutes 

 or until the natural colour of the sections is restored. 



13. Wash well with tap water. 



14. Rinse in distilled water. 



15. Stain in Harris haematoxylin for 2-3 minutes. 



16. Rinse in tap water. 



17. Blue in the lithium carbonate solution for i minute. 



18. Rinse well in distilled water. 



19. Stain in the acid fuchsin solution for 1-2 minutes. 



20. Rinse quickly in tap water. 



21. Immerse in the phosphomolybdic acid solution for 2-5 

 minutes. 



22. Without washing, pass the slides directly into Mallory's 

 Aniline blue - orange G and leave therein for 1-2 hours. 



23. Differentiate with 95% alcohol, controlling by examination 

 under the microscope until the blue granules are intense, but the 

 fuchsinophil granules are clearly visible. 



24. Rinse with absolute alcohol. 



25. Rinse with carbol xylol. 



26. Immerse in two changes of xylol for 10 minutes in each. 



27. Mount in Clearmount of D.P.X. 



Results: 



The nuclei of all cells are stained dark blue black. Granules of 

 basophils, blue. Acidophil granules, brilliant red. Non-granular 

 cytoplasm of chromophobes, light grey. Erythrocytes, orange to 

 red. Bone, intense blue. 



Reference: Gude, William D. (1953), Stain Tech., 28, no. 3, 161-2. 



M 151 



