MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



MALLORY STAIN - HAEMATOXYLIN (Ehrlich) 

 For Negri bodies in sections of brain 



Solutions required: 



A. Ehrlich haematoxyhn. 



B. Orange G. . . . . . . . . 0-5 gm. 



Phosphotungstic acid, saturated 

 aqueous . . . . . . . . 100 ml. 



C. Acid fuchsin . . . . , . 0-5 gm. 

 Phosphotungstic acid . . . . 0-5 gm. 

 Acetic acid 1% . . . . . . 100 ml. 



D. Phosphotungstic acid . . . . 2 gm. 

 Phosphomolybdic acid . . . . 2 gm. 

 Picric acid, saturated, aqueous . . 70 ml. 

 Absolute alcohol . . . . • • 30 ml. 



E. Aniline blue, aqueous . . . . i gm. 



Distilled water . . . . . . 98 ml. 



Glacial acetic acid . . . . . . 2 ml. 



Technique: 



1 . Fix paraffin sections to slides ; dewax and take down to dis- 

 tilled water in the usual way. 



2. Stain in Ehrlich haematoxyhn for five minutes. 



3. Blue in tap water, or in lithium carbonate solution, for two 

 minutes. 



4. Rinse in distilled water. 



5. Stain for one minute in the orange G solution. 



6. Wash in tap water until only the erythrocytes are stained 

 yellow. 



7. Rinse in distilled water. 



8. Stain for ten minutes in the acid fuchsin solution. 



9. Rinse in distilled water. 



10. Differentiate for five minutes in solution D. 



1 1 . Rinse in distilled water. 



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