SECTION TWO 



12. Rinse in i% acetic acid. 



13. Dehydrate, clear and mount. 



Results: 



Negri bodies, purplish red with blue granulations. Cytoplasm 

 of neurones, bluish. Nucleoli, dark purple. Erythrocytes, yellow. 



Reference: Zlotnik, I. (1953), Nature, 172, no. 4386, 962-3. 



MALLORY HEIDENHAIN STAIN (Jane E. Cason's 



modification) 



A rapid one-step method for connective tissue 



Solutions required: 



Phosphotungstic acid crystals A. R. i gm. 



Orange G. . . . . . . 2 gm. 



Aniline blue, water soluble . . i gm. 



Acid fuchsin . . . . • • 3 gni. 



Distilled water . . . . . . 200 ml. 



Technique: 



I . Fix pieces of tissue in Zenker-formol for preference, although 

 Bouin's fluid, formalin and alcohol have been used with success. 



a. Embed in paraffin wax and cut sections 6// in thickness. 



3. Fix sections to slides and remove wax with xylol. 



4. Pass through descending grades of alcohol and if Zenker- 

 formol has been used as the fixative, treat with iodine and sodium 

 thiosulphate as usual to remove mercurial precipitate. 



5. Take down to tap water. 



6. Immerse for five minutes in the staining solution. 



7. Wash in running tap water for three to five minutes. 



8. Dehydrate rapidly through the usual graded alcohols. - 



9. Clear in xylol and mount. 



153 



