SECTION TWO 



8. Stain twelve to twenty-four hours in haematoxylin solution, 

 prepared as above. 



9. Wash in tap water; dehydrate with 95% and absolute 

 alcohol. 



10. Clear in xylol and mount. 



Results: 



Nuclei, centrioles, achromatic spindles, fibroglia, myoglia, 

 neuroglia fibrils, fibrin, contractile elements of striated muscle, 

 blue. Collagen, reticulum, ground substances of cartilage and bone, 

 yellowish to brownish red. Coarse elastic fibrils, faint purple. 



MARSHALL RED - VICTORIA GREEN 

 A general stain, particularly suitable for class work 



Solutions required: 



A. Marshall red, saturated aqueous. 



B. Victoria green saturated in 70% alcohol. 



Technique: 



1. Fix sections to slides; dewax and take down to distilled 

 water in the usual manner. 



2. Stain in the Marshall red solution for twenty minutes. 



3. Rinse in distilled water. 



4. Stain in the Victoria green solution for half an hour, 



5. Rinse in 70% alcohol followed by 90% alcohol. 



6. Dehydrate with absolute alcohol. 



7. Clear in xylol and mount. 



Results: 



Myofibrils, sage green. Nuclei, bright carmine. The results 

 vary somewhat, but the muscle fibres always appear greenish to 

 greenish -grey, while the nuclei are red. White matter of spinal 

 cord, yellowish-green. Cartilage, pink. Retina stands out well 

 as the rods and cones appear bright bluish-green. Erythrocytes 

 unstained. 



Reference: Cannon, H. G. (1941), J. Roy. Micro. Soc.y series III, 61, parts 

 3 and 4. 



