MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Results: 



Nuclei are stained blue; karyosomes, dark blue; plasmosomes, 

 red; basophil cytoplasm, blue; oxyphil cytoplasm and oxyphil 

 granules, red. 



METHYL GREEN 

 For amyloid 



Solution required: 

 Methyl Green i% aqueous. 



Technique: 



1. Fix tissues in absolute alcohol or in io% formalin and cut 

 frozen sections. 



2. Immerse in the methyl green solution for five to ten hours. 



3. Wash well in distilled water. 



4. Mount in neutral glycerine, or in aquamount. 



Results: 



Amyloid is stained reddish violet, while other tissue elements 

 are green. 



METHYL GREEN - PYRONm (Pappenheim-Unna) 



For plasma cells 



Solution required: 

 Methyl Green - Pyronin (Pappenheim-Unna). 



Technique: 



1. Paraffin sections of absolute alcohol-fixed tissues are mounted 

 on slides and brought down to distilled water by the standard 

 method. 



2. Stain in Methyl Green - Pyronin (Pappenheim-Unna) from 

 five to thirty minutes. 



3. Rinse in distilled water; drain and blot carefully. 



4. Dehydrate rapidly in two changes of absolute alcohol. 



5. Clear in xylol; and mount. 



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