SECTION TWO 



Results: 



Plasmosomes and oxyphil constituents of cytoplasm are stained 

 red; chromatin reticulum and karyosomes, bluish green; cyto- 

 plasm of plasma cells, brilliant red ; ground-substance of hyaline 

 cartilage, yellowish red; bacteria, vivid red. 



METHYL VIOLET 6B 

 For amyloid 



Solution required: 

 Methyl Violet 6B aqueous i%. 



Technique: 



1. Material should be fixed in alcohol and embedded in paraffin 

 wax (although frozen sections may be employed). 



2. Paraffin sections are fixed to slides and brought down to dis- 

 tilled water. 



3. Stain for two to five minutes in the methyl violet 6B solution. 



4. Rinse quickly in distilled water. 



5. Differentiate with 1% acetic acid, controlling by examination 

 under the microscope, until the amyloid appears reddish in 

 colour. 



6. Wash in distilled water. 



7. Mount in glycerine jelly. 



Results: 



Amyloid substance is stained red, while cells and nuclei are in 

 varying shades of blue. 



Notes: 



(a) The technique is not suitable for material which has under- 

 gone prolonged fixation in formalin. 



{h) The stain is extracted from the amyloid by alcohol and for 

 this reason glycerine jelly is used as the mountant, thereby obvi- 

 ating the use of alcohol for dehydration. 



159 



