SECTION TWO 



MUCICARMINE - METANIL YELLOW - 

 HAEMATOXYLIN 



For mucin and connective tissue 



Solutions required: 



A. Haematoxylin (Weigert) A. 



B. Haematoxylin (Weigert) B. 



C. Metanil yellow 0-25% aqueous. 



D. Mucicarmine (Southgate). 



Technique: 



1. Fix material in 10% formalin. 



2. Dehydrate, clear; embed in paraffin wax. 



3. Fix sections to slides; de-wax with xylol and pass through 

 the usual descending grades of alcohol. 



4. Rinse with distilled water. 



5. Stain sections for one minute in a freshly prepared mixture 

 consisting of equal volumes of Solution A and B. 



6. Wash in distilled water. 



7. Immerse in Solution C for about two minutes. 



8. Rinse quickly with distilled water. 



9. Immerse in the mucicarmine solution for forty-five minutes. 



10. Rinse quickly with distilled water. 



11. Rinse quickly with 95% alcohol. 



12. Dehydrate rapidly but thoroughly with absolute alcohol. 



13. Clear in xylol and mount. 



Results: 



Mucin is stained red, while connective tissue is yellow, and 

 nuclei are black. 



1 6s 



