SECTION TWO 



Technique: 



1. Material is fixed in absolute alcohol; cleared; and embedded 

 in paraffin wax. 



2. Sections are stained for ten minutes in the above solution. 



3. Wash with distilled water. 



4. Dehydrate by plunging the slide into two or three changes 

 of 95% alcohol. 



5. Pass through absolute alcohol ; then clear in xylol ; mount. 



Results: 

 Mucus is stained blue, while the remainder is colourless. 



NADI REACTION 

 For oxidase granules 



Solutions required: 



A. a-Naphthol . . . . . . i gm. 



Distilled water . . . . . . 100 ml. 



Place in a 250-ml. flask and boil until the a-Naph- 

 thol begins to melt; then add 40% potassium hy- 

 droxide aqueous solution drop by drop until the 

 solution becomes yellowish-blue in colour and the 

 a-Naphthol is still not completely dissolved. 



B. Cold tap water . . . . . . 100 ml. 



*D imethy 1 -p -pheny lenediamine 



base . . . . . . . . 0-5 gm. 



Place the water in a clean amber bottle, open the 

 ampoule by filing a groove at one end then breaking 

 in the usual manner. Tip the contents of the am- 

 poule into the bottle; then replace the stopper and 

 allow the bottle to stand, with occasional shaking, 

 for twenty-four hours. Care should be taken that the 

 Dimethyl-p-phenylenediamine base does not come 

 into contact with the body. 



This solution deteriorates after three or four 

 weeks. 



*Note: This should be purchased in a sealed 

 ampoule. 



N 167 



