SECTION TWO 



D. Naphthol blue black (C.I. No. 246) i gm. 

 Picric acid, satd., aqueous . . 100 ml. 



Technique: 



1. Paraffin sections are fixed to slides and taken down to 70% 

 alcohol in the usual manner. 



2. Stain for six minutes in a freshly prepared mixture consisting 

 of equal parts of solutions of A and B. 



3. Wash in tap water. 



4. Counterstain for five minutes in solution C. 



5. Wash in 1% acetic acid. 



6. Stain in solution D for five minutes. 



7. Rinse in 1% acetic acid for two minutes. 



8. Pass through the usual ascending grades of alcohol dehydrate 

 in absolute. 



9. Clear in Xylol. 



10. Mount in D.P.X. or Clearmount. 



Results: 



Collagen, reticulum and basement membranes, dark green. 

 Smooth muscle, brown. Nuclei, brownish black. 



Reference: Lillie, R. D. (1945), J. Tech. Meth., 25, 47. 



NAPHTHOL GREEN B - HAEMATOXYLE^ (Weigert) 



For connective tissue 



Solutions required: 



A. Weigert's haematoxylin, A. 



B. Weigert's haematoxylin, B. 



C. Eosin, yellowish, 1% in tap water. 



D. Ferric chloride, hydrated 10%. 



E. Naphthol Green B, 1% aqueous. 



F. Equal volumes of acetone and xylol. 



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