SECTION TWO 



Results: 



Collagen fibres, deep blue. Cytoplasm, red. Epithelial cells, 

 centroiles, basal bodies, nuclear structure, brilliant red. Erythro- 

 cytes, yellowish red. Connective tissue, blue. Secretory bodies, 

 varying according to their nature. Slime of goblet cells, azure 

 blue. Connective tissue, wandering cells, smooth and striated 

 muscle, well defined. 



PERIODIC ACID (HOTCHKISS) - CELESTIN BLUE 



For human and animal pituitary glands, demonstrating 

 both muco-protein precursors of the gonadotrophins 



Compared with other methods, finer differences in the cytology 

 of the cyanophils can be appreciated. Cell counts can readily 

 be carried out, and the counts are more accurate, giving more 

 definite and clearer results than those obtained by older methods : 

 for instance cells appearing by Mallory and other histological 

 methods, to be chromophobes, are found to belong to cyanophil 

 series. 



The method has been applied with good cytological results to 

 the hypophysis of sheep and goats. 



Solutions required: 



A. Helly Fixative containing 5% of 

 neutralized formalin. 



B. Lugol's iodine. 



C. Sodium thiosulphate 5% aqueous. 



D. Hotchkiss' periodic Fuchsin. 



E. Hotchkiss' reducing rinse {Solution C as 

 page 184). 



F. Feulgen's Fuchsin. 



G. Celestin blue {Lendrum and Mcfarlane) 

 Celestin blue . . . . . . 0-25 gm. 



Iron alum . . . . . . . . 2*5 gm. 



Distilled water . . . . . . 50 ml. 



Glycerine . . . . . . • • 7 n^» 



181 



