SECTION TWO 



1 6. Wash in running water for ten to thirty minutes. 



17. Stain in the celestin blue solution one half to three minutes. 



18. Rinse in water. 



19. Stain in the toluidine blue (solution H) for one half to three 

 minutes. 



20. Wash in running water for five minutes. 



21. Stain in the phosphomolybdic orange G (solution I) for 

 ten seconds. 



22. Wash in water for five to thirty seconds, until a yellow tinge 

 is just visible to the naked eye. 



23. Dehydrate through the usual graded alcohols. 



24. Clear in xylol and mount. 



Note : In place of Toluidine blue, Iron Haematoxylin may be used 

 at step 19 in which case it will be necessary to differentiate quickly 

 before washing in running water {step 20). 



Results : 



Beta granules in the cyanophils and a number of granules and 

 vesicles in cells which stain as chromophobes by other methods, 

 are magenta to deep red: the colloid is magenta. Alpha granules 

 of acidophils, orange yellow, and erythrocytes are a shade more 

 yellow. 



Nuclei: are blue-black. 



Reference: Pearse, Anthony G. Everson (1950), Stain Tech., 25, 95-102. 



PERIODIC ACID - FEULGEN FUCHSIN (Hotchkiss) 



For staining polysaccharide structures in fixed animal or 



plant tissue preparations 



Solutions required: 



A. Periodic acid 

 Distilled water 

 Sodium acetate M/5 



B. Distilled water 

 Periodic acid 



183 



0*4 gm. 



45 nfil- 

 5 ml. 



10 ml. 

 0-4 gm. 



