SECTION TWO 



6. Wash thoroughly in distilled water. 



7. Dehydrate ; clear and mount in the usual manner. 



Results: 



The following are stained (red) intensely by this reaction: 

 muscle glycogen, liver, hyaluronic acid, gastric mucin, umbilical 

 cord polysaccharide, chitin, crude serum albumin, crude casein, 

 pneumococcus type III polysaccharide, Friedlander type B poly- 

 saccharide, algin, lemon pectin, gum arable, gum tragacanth, gly- 

 cerine, serine, dihydroxyacetone, ribose, arabinose, a-glycero- 

 phosphate, mannitol, tartaric acid, gluconic acid ; while the follow- 

 ing take up the stain with moderate intensity : starch, glucuronic 

 acid, pneumococcus type I polysaccharide, pneumococcus type 

 II polysaccharide; and the following are weakly stained: cellu- 

 lose, crystalline serum albumin, crystalline tgg albumen, glucose, 

 glucosamine, glucose-1-phosphate, galactose, maltose, saccharose, 

 xanthosine, adenosine, muscle adenylic acid and phlorizin. 

 Tryptophane is coloured brown, and the following do not take up 

 the stain: ribonucleic acid, desoxyribonucleic acid, cellobiose, 

 inositol, malic acid and tyrocidine. 



Notes: 



{a) Plant tissues are brilliantly stained, in general revealing 

 cellulose or cellulose-like walls of the individual cells and stored 

 carbohydrate such as starch granules, particularly in the region of 

 the chloroblasts if these are present. The cell walls of freshly cut 

 potato reveal every fold, wrinkle or tear. 



(b) Accumulations of polysaccharides are less common in 

 animal preparations, but mucin, because of its polysaccharide 

 content, is strongly stained. 



(c) If it is desired to demonstrate glycogen^ Solution B should be 

 used in place of Solution A. 



From Archives of Biochemistry, Vol. 16, No. i, pages 131-142 (R. D. Hotch- 

 kiss), by courtesy of Academic Press, Inc., New York, 10, U.S.A. 



PHLOXIN - HAEMATOXYLIN 

 For hyaline, in animal tissues 



Solutions required: 



A. Ehrlich haematoxylin solution. 



B. Phloxin 0.5% in 25% alcohol. 



185 



