SECTION TWO 



3. Fix immediately by immersing in pure methyl alcohol for 

 five to ten minutes. 



4. Rinse in running water; then stain in the phloxin solution 

 from two to five minutes. 



5. Wash in running water; then stain in methylene blue 

 (Loeffler) for ten to thirty seconds. 



6. Decolorize in 80% alcohol; then dehydrate in 95% alcohol 

 and two changes of absolute alcohol. 



7. Clear in xylol and mount in balsam. 



Note: The slides should be handled with forceps throughout to 

 prevent the preparation being spoiled by coming into contact 

 with the fingers. 



Results: 



Pyramidal cells, blue; Negri bodies, bright red to reddish 

 brown. 



PHLOXIN - METHYLENE BLUE - AZUR B 



For normal and pathological animal tissues 



This is a rapid modification of Mallory's original method re- 

 ducing the staining time from one hour or more to one to two 

 minutes, and permitting good staining with formalin fixed tissues 

 which is not possible with Mallory's original method which was 

 designed for Zenker-fixed material. Colophony differentiation is 

 obviated, and Phloxin is not washed out as in the original 

 Mallory technique. 



Solutions required: 



A. Phloxin . . . . . . . . 0-5 gm. 



Acetic acid 0-2% aqueous . . . . 100 ml. 



Filter before use. 



B. Methylene blue . . . . . . 0-25 gm. 



Azur B . . . . . . . . 0-25 gm. 



Borax.. .. .. ., .. 0-25 gm. 



Distilled water . . . . . . 100 ml. 



C. Acetic acid 0-2% aqueous. 



187 



