MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Technique: 



1 . Fix sections to slides : remove paraffin and pass through the 

 usual descending grades of alcohol to distilled water. 



2. Immerse in solution A for one to two minutes. 



3. Rinse well in water. 



4. Stain for one half to one minute in solution B. 



5. Destain partially in solution C. 



6. Differentiate in three washes of 95% alcohol. 



7. Dehydrate with two changes of absolute alcohol. 



8. Clear in two changes of xylol. 



9. Mount in synthetic medium (Permount, D.P.X. or Clear- 

 mount, etc.) 



Results: 



Nuclei and bacteria are stained blue with some metachromasia. 

 Collagen and other tissue elements are bright pink to red. Erythro- 

 cytes, bright scarlet. 



Reference: Thomas, John T. (1953), Stain Tech., 28, no. 6. 311 — 312. 



PHLOXIN - TARTRAZINE 



(A. C. Lendrum's technique) 



A general histological stain and for the demonstration of 



inclusion bodies 



Note: This technique, in which use is made of a stable phloxine 

 solution, and which affords the advantage of brilliant demon- 

 stration of certain inclusion bodies, is superior to Masson*s 

 erythrosin-saffron, which is less readily prepared and which 

 deteriorates fairly rapidly. 



Solutions required: 



A. Haemalum (Mayer). 



B. Calcium chloride 0-5% aqueous. 100 ml. 

 Phloxine . . . . . . . . 0-5 gm. 



C. Tartrazine, saturated in cellosolve. 



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