SECTION TWO 



QUINCKE REACTION 

 For haemosiderin 



Solutions required: 



A. Ammonium sulphide solution, 

 concentrated . . . . . . i volume 



Absolute alcohol . . • • 3 volumes 



B. Basic fuchsin 0-5% in 50% alcohol. 



Technique: 



1. Tissues are fixed in neutral formalin 10%, or in absolute 

 alcohol, and embedded in paraffin wax or Celloidin in the usual 

 manner. 



2. Bring sections down to distilled water, then immerse them 

 from two to forty-eight hours in the ammonium sulphide solution. 



3. Rinse thoroughly in distilled water. 



4. Counterstain in the basic fuchsin solution for five to twenty 

 minutes. 



5. Wash in water; drain well; rinse in 80% alcohol. 



6. Differentiate and dehydrate in absolute alcohol; clear in 

 xylol and mount in Canada balsam or in Cristalite. 



Results: 



Haemosiderin, dark brown to black. 



RHODAMINE B - ANILINE METHYLENE BLUE 



For splenic and lymphoid tissues 



Solutions required: 

 A. Methylene Blue, 2% alcoholic 



Aniline water . . ^ . . 

 Distilled water 



B. Rhodamine B 1% aqueous 

 Distilled water 



C. Solution A (above) 

 Solution B (above) 



193 



10 ml. 

 15 ml. 

 30 ml. 



2-5 ml. 

 47-5 ml. 

 3 volumes 

 7 volumes 



