SECTION TWO 



2. Sections are immersed for a second in 70% alcohol; then 

 stained for two to five minutes, in the Scarlet R solution. 



3. Wash quickly in 70 % alcohol, and transfer to distilled water. 



4. Counterstain with Ehrlich or Delafield haematoxylin. 



5. Wash well in tap water; mount in glycerine or glycerine jelly. 



Results: 



Nuclei, blue. Fat, orange to red. Cholesterol, red. Normal 

 myelin, unstained. Fatty acids, unstained. 



SILVER CARBONATE - ORCEIN - ANILINE BLUE - FAST 



GREEN 



For demonstrating reticulin, elastin and collagen in the 



same tissue sections 



Solutmis required: 



A. Celloidin 0-5% in equal vols, of Ether and 

 Absolute Alcohol. 



B. Pot. Perman. 0-25% aqueous. 



C. Oxalic Acid 5% aqueous. 



D. Silver carbonate^ Hortega {Foot's modification) 

 Silver nitrate 10% aqueous . . 10 ml. 

 Lithium carbonate, saturated 



aqueous . . . . . . . . 10 ml. 



Shake well; then allow to stand for ten 

 minutes or so in a 25 ml. measuring cylinder. 



Pour off the supernatent fluid, then transfer the precipitate to 

 a 100 ml. measuring cylinder, and add about 75 ml. distilled water, 

 shake well ; allow to settle ; then pour off the fluid and add a second 

 lot of distilled water. This process should be repeated three or 

 four times. 



Finally add 25 ml. of distilled water to the precipitate and add 

 28% ammonia solution drop by drop until the precipitate is almost 

 dissolved. 



Make up to 100 ml. with 90% alcohol. 



Filter and warm to 50° C. for 15 minutes before using. 



p 199 



