' MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



E. Neutral Formalin 40% . . . . 20 ml. 



Distilled water . . . . . . 80 ml. 



Buffer to pH 7-0. 



F. Gold chloride 0*2% aqueous. 



G. Sodium Thiosulphate 5%. 



H. Orcein 1% in 70% alcohol . . 100 ml. 



Hydrochloric acid, cone. . . . . i ml. 



Picro Aniline Blue 

 I. Aniline blue aqueous . . . . o*i gm. 



Picric acid, saturated aqueous . . 100 ml. 



or: 



J. Picric acid, saturated aqueous . . 100 ml. 

 Fast Green FCF . . . . . . 0*2 gm. 



Technique: 



1. Material should be fixed in 10% formalin and embedded in 

 paraffin wax by the standard technique. 



2. Sections 4 to 5/i, in thickness are fixed to slides, dewaxed and 

 immersed in xylol for five minutes. 



3. Wash and immerse in absolute alcohol for two minutes. 



4. Immerse in solution A for five minutes (Celloidin). 



5. Drain slides for one minute. 



6. Immerse in 80% alcohol for five minutes. 



7. Rinse in water. 



8. Immerse in solution B (Pot. perman.) for five minutes. 



9. Rinse in water. 



10. Immerse in solution C (oxalic acid) for five minutes. 



11. Rinse in tap water. 



12. Wash in distilled water. 



13. Immerse in the silver carbonate solution in an oven 

 at 50° C. for ten to fifteen minutes. (Soln. D). 



14. Rinse in distilled water. 



15. Immerse in solution E (20% neutral formalin) for five 

 minutes. 



200 



