SECTION TWO 



1 6. Rinse in tap water. 



17. Tone in solution F (Gold chloride) for five minutes. 



18. Rinse in tap water. 



19. Immerse in solution G for two minutes. 



20. Rinse in tap water. 



21. Stain in solution H (Orcein) for ten to fifteen minutes in 

 an oven at 37° C. or for one hour at room temperature. 



22. Rinse in 70% alcohol, followed by tap water. 



23. Stain in solution I (Aniline blue) for twenty to forty seconds 

 or solution J (Fast green) for ten to twenty seconds. 



24. Wash with 95% alcohol for six to eight seconds. 



25. Rinse briefly with absolute alcohol. 



26. Rinse with a mixture consisting of equal volumes of xylol 

 and absolute alcohol until clear. 



27. Rinse with several changes of xylol, and mount. 



Results: > 



With Picro aniline blue 



Elastic fibres reddish brown 



Collagen blue 



Reticulum black 



Muscle blue-green 



Nuclei tan to brown 



Erythrocytes yellowish tan 



Cytoplasm pale blue 



With Picro fast green 



Elastic fibres orange to reddish brown 



Collagen blue-green 



Reticulum black 



Muscle green 



Nuclei tan to brown 



Erythrocytes yellow to orange 



Cytoplasm light green 



Reference: Lewis, Ann L., and Jones, Russell S., (1951), Stain Tech., 26, 

 85-7. 



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