SECTION TWO 



10. Fix sections to slides ; de-wax and pass through descending 

 grades of alcohol down to distilled water. 



11. Tone sections on slides by immersing in the gold chloride 

 solution for five to ten minutes. 



12. Wash quickly in distilled water. 



13. Fix in 5% sodium thiosulphate (Solution E) for ten to 

 fifteen minutes. 



14. Wash thoroughly in distilled water. 



15. Counterstain with paracarmine for about ten minutes. 



16. Rinse with 90% alcohol, followed by absolute alcohol. 



17. Clear in xylol and mount. 



Results: 



Golgi apparatus stained black while cells are pink or red. 



SILVER NITRATE - HYDROQUINONE 



For the detection of gold in filxed tissues of experimental 



animals 



Notes: 



(a) The following technique must be carried out in the dark- 

 room. 



(b) Fixatives containing metals must be avoided. 



{c) The use of metal instruments in handling the sections must 

 be avoided. 



Solutions required: 



A. Gum acacia 10% aqueous, filitered 100 ml. 

 Silver nitrate, A.R. grade . . 2 gm. 



Note: This solution should be prepared immedi- 

 ately before use. 



B. Gum acacia 10% aqueous, filtered 100 ml. 

 Hydroquinone . . . . . . i gm. 



Note: This solution should be prepared the day 

 before it is required for use. 



203 



