SECTION TWO 



6. Transfer the sections to distilled water for three to five 

 minutes. 



7. Counterstain with carmalum for about three minutes. 



8. Wash with distilled water. 



9. Mount in glycerine jelly. 



Results: 



Lipid substances or particles are stained blue-black to black. 

 Nuclei, red. 



Reference: Chiffelle, T. L., and Putt, F. A. (195 1), Stain Tech., 26, no. i, 

 pages 51-6. 



SUDAN BLUE 

 For demonstrating degenerated myelin 



Solution required: 



Sudan blue, saturated in 50% 

 alcohol. 

 Technique: 



1. Tissues should be fixed for at least three days in 10% neutral 

 formalin. 



2. Frozen sections are soaked one minute in 50% alcohol; then 

 one minute in 70% alcohol ; then stained from fifteen minutes to 

 several hours in a saturated solution of Sudan blue in 50% alcohol. 



3. Rinse for a few minutes in 50% alcohol; then in distilled 

 water. 



4. Counterstain in carmalum for three to five minutes. 



5. Rinse in water; mount in glycerine jelly. 



Result: 



Degenerated myelin, blue. 



SUDAN BROWN 



For fat and for acute fatty degeneration not shown by 



Scarlet R 



Solutions required: 



A. Stock solution of Sudan brown 



saturated in isopropyl alcohol. 



B. Mayer's acid haemalum. 



209 



