SECTION TWO 



C. Iron alum . . . . . . 0-5% aqueous 



D. Sudan 2, saturated in isopropyl 



alcohol . . . . . . . . 30 ml. 



Distilled water . . . . . . 20 ml. 



Mix well and allow to stand for ten minutes before 

 use. 



Note: This solution deteriorates within three to 

 four hours. 



Technique: 



1 . Formalin-fixed frozen sections are stained forty minutes with 

 Solution A at 56° C. in a covered dish in an oven. 



2. Rinse in water and differentiate for one hour with Solution B. 



3. Rinse in distilled water and immerse for ten minutes in 

 Solution C. 



4. Stain ten to twenty minutes in Solution D ; then float out in 

 water. 



5. Mount in Apathy's gum syrup or in Aquamount. 



Results: 



Normal myelin, blue-black; nerve cells, grey; nuclei, deeper 

 grey ; red corpuscles, yellow to black ; fats, orange yellow. 



THIONIN (Ehrlich) 

 For mucin 



Solution required: 



Thionin (Ehrlich), saturated, 



aqueous . . . . . . 0-5 ml. 



Distilled water . . . . . . 10 ml. 



Technique: 



I. Tissues should be fixed in Zenker's fluid, washed in running 

 water, dehydrated, cleared, embedded and sectioned in the usual 

 manner. 



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