SECTION TWO 



THIONIN (Ehrlich) 

 For nerve cells and fibre tracts 



Solution required: 



Stain and fixative combined: 

 Thionin 0-5% in io% formalin. 



Technique: 



1. Tissues are fixed and stained simultaneously by immersing 

 in the above solution from a few days to three months. 



2. Wash well in running water. 



3. Dehydrate in ascending grades of alcohol in the usual man- 

 ner. 



4. Clear ; embed in paraffin wax or in Celloidin. 



5. Fix sections to slides and mount in Cristalite. 



Results: 



Cell bodies are stained blue, while the fibre tracts are red. 



THIONIN (Ehrlich) 

 For demonstrating malignant cells in biopsy material 



Solution required: 

 Thionin 1% aqueous. 



Technique: 



1. Stain frozen sections for ten to sixty seconds in the thionin 

 solution. 



2. Rinse in water. 



3. Mount in tap water or in Aquamount. 



Results: 



Nuclei, blue to purple. Collagen, red. Elastic tissues, green. 



213 



