MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



TOLUIDINE BLUE 

 For mucus 



Solution required: 

 Toluidine Blue 1-5% aqueous. 



Technique: 



1. Formalin-fixed material is embedded in paraffin wax in the 

 usual manner. 



2. Bring sections down to distilled water then stain for one or 

 two minutes in the toluidine blue solution. 



3. Wash with distilled water; drain well, then plunge the slide 

 into two changes of 95% alcohol. 



4. Dehydrate by immersing in two changes of acetone; then 

 clear in xylol and mount. 



Results: 



Mucus, reddish violet; nuclei, blue; erythrocytes, yellow to 

 greenish yellow. 



TRICHROME STAIN 

 (G. Gomori) 



A. Delafield or Ehrlich Haematoxylin. 



B. Lithium carbonate 1% aqueous. 



C. Alcohol 70% 97 ml. 



HCl, concentrated . . . . • • 3 nil- 



D. Picric acid 1% in 50% alcohol. 



E. Phosphotungstic Acid 3%. 



F. Light green, or Fast green FCF, or 

 Aniline blue . . . . . . . . 0-5 gm. 



Neoponceau (Michrome) . . . . 1-5 gm. | 



G. Solution F . . . . . . . . i volume 



Acetic acid 2% . . . . 3 to 4 volumes 



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