SECTION TWO 



Technique: 



1. Fix tissues in Bouin or io% Formalin. 



2. Stain sections in the Haematoxylin solution for ten minutes. 



3. Blue in the lithium carbonate solution. 



4. Differentiate, if necessary, with the HCl alcohol for pre- 

 dominance of the green or blue shades, or in picric alcohol for 

 predominance of red shades, in the final picture. 



5. Immerse the preparation in 3% phosphotungstic acid for 

 ten to fifteen minutes. 



6. Wash gently under the tap for one minute. 



7. Stain in solution G for five to twenty minutes. 



Note: The time is not critical although results will be slightly 

 different. 



8. Rinse in 2% acetic acid. 



9. Dehydrate, clear and mount. 

 Results: 



Nuclei, blue. Cytoplasm, muscle fibres, red cells, etc., in shades 

 of red. Connective tissue green or blue. 



From personal communications with Professor G. Gomori of the 

 Department of Medicine ^ University of Chicago, U.S. A., to whom 

 I am indebted for permission to include this hitherto unpublished 

 technique. 



Note: Professor Gomori has used Woodstain scarlet, which is 

 not available under that name in Britain. The British equivalent 

 is Neoponceau and I feel that this synonym is more suitable in 

 this case as the name Woodstain scarlet, indexed in literature on 

 general biology, might suggest a botanical stain for woody tissues. 



TRICHROME STAIN (Masson), Modified 



For epithelium, pituitary and thyroid glands, nerve (normal 



and tumour), etc. 



Solutions required: 



A. Regaud's haematoxylin. 



B. Picric acid saturated in 95% alcohol 20 ml. 

 Alcohol 95% . . . . . . 10 ml. 



Q 215 



