MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



C . Violamine-acid fuchsin : 



Violamine, R . . . . . . 0-7 gm. 



Acid fuchsin . . . . . . 0-3 gm. 



Glacial acetic acid . . . . i ml. 



Distilled water . . . . . . 100 ml. 



D. Acetic fast green 



Fast green, F C F . . • • 3 gm- 



Glacial acetic acid . . . . 2 ml. 



Distilled water . . . . . . 100 ml. 



Technique : 



1 . Tissues are fixed in Bouin, Regaud, Zenker or formalin, and 

 embedded in paraffin wax. 



2. Mordant sections on slides with 5% iron alum previously 

 heated to 45° C. 



3. Wash with tap water; stain for five minutes in Regaud's 

 haematoxylin ; then rinse with 95% alcohol. 



4. Differentiate with picric alcohol; then wash with running 

 tap water. 



5. Stain for five minutes in Ponceau-acid fuchsin; then wash 

 with distilled water. 



6. Differentiate for five minutes in 1% phosphomolybdic acid; 

 then without rinsing: 



7. Flood the slide with solution D and leave for five to ten 

 minutes. 



8. Rinse with distilled water; then return to 1% phospho- 

 molybdic acid for five minutes. 



9. Leave in 1% acetic acid for five minutes. 



10. Dehydrate in 95% alcohol, followed by absolute alcohol; 

 clear in xylol and mount. 



Results: 



Nuclei, black. Argentaffin granules, black or red. Cytoplasm, 

 vermilion. Collagen, green. NeurogUa fibrils, vermilion. Mucus, 

 green. Keratin, vermilion. Intercellular fibrils, vermilion. Golgi 

 apparatus, clear. 



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