SECTION TWO 



plasmasomes, red; cytoplasm, blue to violet; granules of the 

 neutrophil leucocytes, sky blue; elastic fibres, red; collagen fibres, 

 blue. 



WEIGERT - FRENCH ELASTIN STAIN 

 (Moore's modification) 



This modification, which is due to G. W. Moore, of the Central 

 Histological Laboratory, Archway Hospital, London, gives greater 

 selectivity than either Sheridan or Weigert elastin stains, and 

 consistently excellent results are obtained provided the stain is 

 properly prepared. 



Solutions of Moore's elastin stain will keep for several years 

 without deterioration. 



The dry stain requires time and great care for its preparation; 

 the majority of workers will, no doubt, wish to purchase the stain 

 ready for use, but for those who have the time and prefer to pre- 

 pare the stain themselves, the method is given below. 



Preparation of the dry stain: 



A. Ferric chloride, anhydrous, A.R. 



grade . . . . . . . . 30 gm. 



Distilled water . . . . . • 65 ml. 



Dissolve; then make up the vol- 

 ume to 100 ml. with distilled water. 



Note: This solution must be freshly prepared. 



Note: The water must be heated to about 95° C. 

 in a large evaporating basin, the dyes and dextrin 

 added and stirred until dissolved. The resorcin is 

 then added and the solution raised to boiling point 

 with constant stirring. 



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