SECTION TWO 



Technique: 



1. Immerse sections in the picric acid solution for two minutes. 



2. Wash thoroughly in running tap water. 



3. Stain for five to seven minutes in Weigert's haematoxylin A. 



4. Rinse in water and stain for three to five minutes in the 

 Ponceau S solution. 



5. Wash in water. 



6. Immerse in Weigert's haematoxylin B. 



7. Wash thoroughly in water. 



8. Stain for three to five minutes in the wool green solution. 



9. Decolorize for two minutes with 1% acetic acid. 



ID. Pour off excess acid; rinse well in distilled water; drain and 

 blot carefully. 



1 1 . Rinse well with acetone. 



12. Rinse with two washings of Solution F. 



13. Clear in xylol and mount. 



Results: 



Muscle and cytoplasm, red. Connective tissue and basement 

 membranes, green to dark blue. 



WRIGHT'S STAIN 



For general differentiation of blood corpuscles; for 

 malarial parasites; trypanosomes, etc. 



This stain is extensively used in America instead of Leishman 

 stain which is preferred by British workers. 



Solutions required: 

 Formol-saline^ neutral^ buffered: 



A. Formalin (40% formaldehyde) . . 100 ml. 



Sodium chloride, A.R. . . . . 8-5 gm. 



Distilled water . . . . . . i litre 



Acid sodium phosphate, mono- 

 hydrate . . . . . . • . 4 gm. 



Anhydrous disodium phosphate 6-5 gm. 



225 



