SECTION THREE 



3. Cover the section with a few drops of the sodium molybdate 

 and warm gently for a few minutes. 



Results: 



A deep blue colour turning to violet after a few minutes indicates 

 the presence of tyrosine. 



(c) STAINING TECHNIQUES 



ACID FUCHSIN - AURANTIA 



For di£Ferentiating between bacteria and mitochondria in 



sections of infected tissue 



Technique: 



1 . Kill and fix for twenty-four hours in Solution A. 



2. Wash for twenty-four hours in running water; then de- 

 hydrate and embed. Sections should be cut as thinly as possible. 



3. After removing the paraffin wax from slides they are dipped 

 into a very thin solution of Celloidin in equal parts of absolute 

 alcohol and ether; then passed through absolute, 90%, 70% 

 alcohol to distilled water. 



4. Stain in Solution B, heated to about 80° C, for a few minutes, 

 then wash in running water. 



247 



