SECTION THREE 



10. Dehydrate with absolute alcohol. 



1 1 . Clear in xylol, and mount. 



Results: 



Mitochondria are stained red, while starch grains are blue. 

 Note: This is an adaptation of one of Volkonsky's techniques. 



Reference: Milovidov (1928), Arch. Anat. Micros., 24, 9. 



ANILINE HYDROCHLORIDE 



A simple and rapid method of demonstrating lignified 



tissues 



Solution required: 



Aniline hydrochloride 10% 

 aqueous, freshly filtered before 

 use. 



Technique: 



1. Place sections on slides and cover with a few drops of the 

 aniline hydrochloride solution, and allow this reagent to act for 

 about five minutes. 



2. Pour off excess ; place a drop of glycerine on the section and 

 cover with a coverglass. 



Results: 



Lignified tissues are stained yellow while the other tissues remain 

 unstained. 



BASIC FUCHSIN, AMMONIACAL 

 For lignified walls and cutin 



Solution required: 



A. Basic fuchsin, 10% alcoholic. 



B. Strong ammonia solution . . 25 ml. 



Note: The fuchsin solution is added drop by drop 

 to the ammonia until a yellowish colour is produced. 



249 



