SECTION THREE 



CYANIN - ERYTHROSIN 

 A botanical stain for cellulose and lignified tissues 



Solutions required: 



A. Cyanin, o*i% in 50% alcohol. 



Note: This is a very costly stain. 



B. Erythrosin 1% in 70% alcohol. 



Technique: 



1. Stain sections from five to twenty minutes in the cyanin 

 solution, placing the slide under a petri dish lid, or an evaporating 

 basin lined with a piece of damped filter paper to prevent evapora- 

 tion of the stain. 



2. Wash rapidly with 50% alcohol. 



3. Stain from half to one minute in the erjrthrosin. 



4. Rinse quickly in 50%. 



5. Rinse quickly in 95% alcohol. 



6. Dehydrate by rinsing quickly in two changes of absolute 

 alcohol. 



7. Clear in xylol, and mount. 



Results: 



Lignified tissues are stained blue, while cellulose tissues are red. 



DELAFIELD HAEMATOXYLIN - CELLOSOLVE 

 For botanical tissues, particularly for cell walls 



Solutions required: 



A. Delafield haematoxylin. 



B. Alcohol 70% . . . . . . 99*5 ml. 



Ammonia solution (sp. gr. o-88o) 0-5 cc. 



Technique: 



I. Fix sections to slides and stain in Delafield haematoxylin for 

 three to five minutes. 



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