MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



G. Xylol . . . . . . . . 90 ml. 



Absolute alcohol . . . . . . 10 ml. 



Technique: 



1. Pass sections through descending grades of alcohol to dis- 

 tilled water. 



2. Mordant in solution A for thirty minutes. 



3. Rinse in distilled water. 



4. Stain in solution B for twelve hours. 



5. Rinse in distilled water. 



6. Differentiate in 2% iron alum solution. 



7. Wash in running water for one hour. 



8. Dehydrate in 25%, 50%, 70%, 90% and two changes of 

 absolute alcohol. 



9. Stain in the aniline blue solution for ten minutes. 



10. Remove excess aniline blue with absolute alcohol. 



11. Rinse in solution E. 



12. Clear in solution F for ten minutes. 



13. Rinse in solution G. 



14. Rinse in two changes of xylol and mount in Canada balsam 

 in xylol, Clearmount, Cristalite or D.P.X. 



Results: 



Nuclei and cytoplasm are well stained by the haematoxylin, 

 while cell walls are stained very satisfactorily by the aniline blue. 



Note: 



(a) The success or failure of the method is said to depend on 

 the quality of the aniline blue. - 



[h) Ilford Special Rapid Panchromatic plates were used for the 

 preparation of the photomicrographs which are reproduced in the 

 original paper. 



Reference: Vaughan, J. G. (1955), Stain Tech., 30, no. 2, 79-82. 



258 



