SECTION THREE 



LIGNIN PINK 

 A non-fading stain which is specific for lignin 



The stain gives constant results with reasonably thin sections 

 and is particularly suitable for routine or elementary classwork. 



Overstaining with Lignin Pink is impossible, and it will not wash 

 out with alcohol. 



In combination with chlorazol black it offers a simple double 

 staining technique as follows : 



Technique: 



1. Reasonably thin sections are fixed to slides and immersed in 

 the staining solution for twenty to twenty-five minutes. 



2. Rinse in distilled water. 



3. Rinse in 70% alcohol. 



4. Rinse in 90% alcohol. 



5. Dehydrate with absolute alcohol. 



6. Clear in xylol and mount. 



Results: 



Lignin stands out, stained bright carmine colour, against the 

 surrounding tissues which are stained black. 



Reference: Cannon, H. G. (1941)^^. jR. Mic. Soc, series III, 61, parts 3 and 4. 



MAGDALA RED - FAST GREEN 



A differential stain for parasite and host tissues in botanical 



material 



Solutions required: 



A. Magdala red 2% in 80% alcohol (original) 



B. Fast Green FCF-Clove Oil. 



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