SECTION THREE 



B. Cotton blue 4B . . . . . . 0-02 gm. 



Safranin . . . . . . . . o-io gm. 



Solution A . . . . . . . . 100 ml. 



C. Safranin 0*25% in clove oil,. 



Technique: 



1. Embed material in paraffin wax by the standard technique. 



2. Fix sections to slides and remove paraffin wax with xylol as 

 usual. 



3. Wash well with absolute, followed by 90% alcohol. 



4. Immerse in solution A for ten to fifteen minutes. 



5. Transfer to solution B and leave therein for two hours. 



6. Differentiate in solution A. 



7. Wash with absolute alcohol. 



8. Immerse in solution C for twenty to thirty minutes. 



9. Differentiate in clove oil. 



10. Clear in xylol, and mount in Canada balsam in xylol or in 

 Cristalite, or in Emexel mountant. 



Results: 



Host tissue is stained red ; Myelium blue. 



Reference: (1928), Lepik. Phtopath., 18, 869. 



SAFRANIN - FAST GREEN IN CELLOSOLVE 



A rapid, non-fading stain for botanical tissues in place 

 of Safranin -light green -clove oil 



Solution required: 

 2 % Safranin - Fast Green FCF in cellosolve. 



Technique: 



1. Stain sections for five to ten minutes. 



2. Rinse in cellosolve. 



4. Mount in Canada balsam or in CristaHte. 



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