MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Results: 



Nuclei, chromosomes, lignified and cutinized cell walls, brilliant 

 red. Cytoplasm and cellulose cell wall, brilliant green. In some 

 cell walls at certain developmental or formative stages, portions 

 will be stained less sharply by safranin, and other portions weakly 

 by the fast green. 



SAFRANIN - FAST GREEN, FCF IN CELLOSOLVE 



A rapid non-fading stain for botanical tissues in place of 

 Safranin - light green - clove oil 



Solution required: 



Safranin - light green in Cellosolve. 

 Technique: 



1. Stain sections in the safranin - light green - cellosolve for 

 five to ten minutes. 



2. Rinse in Cellosolve. 



3. Mount in Canada balsam or in Cristalite. 



Results: 



Lignified tissues are stained red, while unlignified tissues and 

 cytoplasm are blue-green. Nuclei, red. Chloroplasts, pink to red. 



SAFRANIN - LIGHT GREEN - CLOVE OIL 

 A general stain for botanical tissues 



Solutions required: 



A. Safranin 1% in 50% alcohol. 



B. Light Green - clove oil. 



Technique: 



1. Stain section in the safranin solution for ten minutes. 



2. Rinse in two changes of 50% alcohol for thirty seconds in 

 each. 



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