MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Results: , 



With Scarlet R: Nuclei, blue. Fat, orange to red. Cholesterol, j 



red. Fatty acids, unstained. i 



With Sudan 3 .* Nuclei, blue. Fat, yellow to orange. Cholesterol, \ 

 orange. Fatty acids, unstained. 



SCARLET R 

 For staining fat in plant tissues 



Solutions required: ^ 



A. Scarlet R, saturated in 70% alcohol. j 



B. Delafield or Ehrlich haematoxylin. 



Technique: 



1. Fix tissues in 10% formalin. 



2. Sections are immersed for a second in 70% alcohol; then 

 stained for two to five minutes in the Scarlet R. 



3. Wash quickly and transfer to distilled water. 



4. Counterstain with Ehrlich or Delafield haematoxylin. 



5. Wash well in tap water; mount in glycerine or glycerine jelly. 



Results: 



Fat, orange to red. Cholesterol, red. Nuclei, greyish blue. 

 Fatty acids, unstained. 



SCHULZE SOLUTION (Chlor Zinc Iodine) 

 For cell walls, proteins and starch 



1. Place sections on slides and pour on a few drops of Schulze 

 solution. 



2. Cover with a coverslip and examine under the microscope. 



Results: 



Starch, blue. Proteins, brown. Cellulose walls, violet. Ligni- 

 fied walls, yellow. Cutinized and suberized walls, yellow to brown. 



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