ACID FUCHSIN - TOLUIDINE BLUE - AURANTIA 



For mitochondria 



E. Toluidine blue 0-5% aqueous. 



F. Aurantia 0-5% in 70% alcohol. 



Technique: 



1. Tissues are fixed in Champy's Fluid for twenty-four hours; 

 then washed in running water for at least an hour. 



2. Immerse in Solution B for twelve to twenty hours ; then wash 

 in distilled water for thirty minutes. 



3. Mordant in Solution C for three days; then wash in running 

 water for twenty-four hours. 



4. Dehydrate ; clear ; embed in paraffin wax. 



5. Sections, 3 to 5yW in thickness are taken down to water in the 

 usual manner ; then stained for six minutes by flooding the slide 

 with Solution D, and heating gently till vapour rises. 



6. Rinse in distilled water; then counterstain in Solution E. 



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