SECTION FOUR 



ALIZARIN RED, S 

 For mitochondria, cell inclusions, etc. (Benda's method) 



Solutions required: 



Technique: 



1. Fix for eight days in Solution A. 



2. Wash for one hour in water; then transfer to Solution B 

 for twenty-four hours. 



3. Transfer to Solution C for twenty-four hours. 



4. Wash for twenty-four hours in water. 



5. Dehydrate; clear; embed in paraffin wax. 



6. Mordant sections on slides for twenty-four hours with Solu- 

 tion D ; then rinse in water. 



7. Stain for twenty-four hours in Solution E. 



8. Rinse with water; flood slides with Solution F and warm 

 gently until vapour is given off; or stain at room temperature for 

 twenty-four hours. 



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