( 



MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



9. Rinse in water; then differentiate for two to three minutes 

 in 30% acetic acid until the nuclei appear reddish. 



10. Wash in running water for five to ten minutes ; blot dry. 



11. Dip into absolute alcohol; then pass through bergamot oil 

 into xylol and mount. 



Results: 



Mitochondria, violet. Chromatin and archoplasm, brownish 

 red. Certain secretion granules, pale violet. Centrosomes, reddish 

 violet. 



ANILINE FUCHSIN - IODINE GREEN 

 For mitochondria 



Solutions required: 



A. Acid fuchsin aqueous 10% . . 90 ml. 

 Aniline oil. . . . . . . . 10 ml. 



Mix well by shaking at intervals over a period of 

 several hours ; then filter. 



Note: This solution deteriorates after three or 

 four weeks. 



B. Potassium permanganate 1% aqueous. 



C. Oxalic acid 5% aqueous. 



D. Iodine green 1% aqueous. 



Technique: 



1. Small pieces of tissue are fixed in Regaud's Fluid for four 

 days. 



2. Transfer to 3% aqueous potassium dichromate for eight days 

 changing the solution at intervals of two days. 



3. Wash in running water overnight. 



4. Dehydrate in alcohol as usual and clear in xylol. 



5. Embed in paraffin wax; cut sections not more than 5/^ in 

 thickness. 



6. Fix sections to slides; pass through xylol and descending 



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