SECTION FOUR 



grades of alcohol down to distilled water in the usual manner; 

 then blot carefully. 



7. Place the slides, sections facing upwards, on the corner of a 

 tripod; flood the preparation with aniline fuchsin (Solution A) 

 and heat gently with a small bunsen flame until vapour rises. 



8. Remove the flame and allow the stain to act for five to ten 

 minutes. 



9. Pour oflF the excess stain and drain for a few seconds. 



10. Rinse well in distilled water. 



11. Immerse in the potassium permanganate for five seconds; 

 then pour off^ excess. 



12. Immerse in the oxalic acid solution for five seconds; then 

 pour off excess. 



13. Wash with distilled water; then stain with the iodine green 

 solution for five to ten seconds. 



14. Pour off excess stain; drain for a few seconds; dehydrate 

 quickly with 95% and absolute alcohol. 



15. Clear in xylol; mount in Cristalite. 



Results: • 



Mitochondria, crimson. Nuclei, green. 



ANILINE FUCHSIN - PICRIC ACID (Altmann) 



For mitochondria 



Solutions required: 



A. AltmanrCs Fluid: 



Potassium dichromate 5% aqueous i volume 

 Osmic acid 2% aqueous . . i volume 



Note: Although the^ penetration power of this 

 fi[xative is poor, it is very satisfactory for surface 

 fixation. 



B. Acid fuchsin . . . . . . 20 gm. 



Aniline water . . . . • • 95 nal- 



C. Picric acid saturated in absolute alcohol. 



293 



