MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



FEULGEN STAIN 

 For mitosis in plant cells 



Solutions required: 



A. Feulgen's fuchsin. 



B. Potass, metabisulphite io% . . 5 ml. 



N/i HCl 5 ml. 



Distilled water . . . . . . 100 ml. 



C. Fast green FCF 0-5% in 70% 



alcohol. 

 Technique: 



Tissues should be fixed in Flemming and embedded in paraffin 

 wax. 



1. Sections are brought down to distilled water. 



2. Rinse in N/i HCl; then transfer to N/i HCl at 60° C. for 

 four to five minutes; afterwards rinsing in cold N/i HCl. 



3. Rinse in distilled water; then stain for three to four hours 

 in Solution A. 



4. Drain and immediately transfer to a stoppered jar containing 

 Solution B for ten minutes; then transfer to a second jar of Solu- 

 tion B for ten minutes, followed by ten minutes in a third jar. 



Note: The jars must be kept closed. 



5. Rinse in distilled water; then counterstain for one half to 

 one minute in Solution C. 



6. Dehydrate; clear and mount. 



Results: 



Chromosomes, reddish violet. 



GENTIAN VIOLET - PICRIC ACID - IODINE 

 For chromosomes in plant cells 



300 



