SECTION FIVE 



5. Differentiation of Nerve Tissues 



Method A. Solution required: 



Acridine yellow Hi 07 aqueous o- 1%. 



Technique: 



1. Sections are stained with the acridine yellow for two minutes. 



2. Pour off excess stain and rinse with distilled water. 



3. Dehydrate in the usual manner; clear in xylol; mount in 

 Fluormount. 



Results: 



Neurophile nerve tissue appears light blue, while cortical nerve 

 tissue is light yellow to orange. 



Method B. Solution required: 



Acridine orange o-i% aqueous. 



Technique: 



1. Sections are stained with the acridine orange solution for 

 two minutes. 



2. Pour off excess stain, rinse with distilled water. 



3. Dehydrate as usual, clear in xylol in Fluormount. 



Results: 



Neurophile is bluish-grey while cortical tissue is brownish 

 orange. 



Method C. Solution required: 



Acriflavine o-i% aqueous. 

 Technique: 



1. Stain with the acriflavine solution for two minutes; then 

 pour off excess and rinse in water. 



2. Dehydrate rapidly ; clear in xylol and mount in Fluormount. 



Results: 



Neurophile is blue, while cortical tissue appears brownish 

 yellow. 



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